Acute oral toxicity and anti-inflammatory evaluation of methanolic extract of Rotula aquatica roots in Wistar rats

ABSTRACT

Background: The plant Rotula aquatica Lour. was traditionally well known due to its large number ofpharmacological action and medicinal uses. The plant is a necessary component of many Ayurvedic drugpreparations since historical times. It is widely used as a crucial ancient drug for kidney and bladderstones.Objectives: The main objective of the study was to evaluate the acute toxicity and anti inflammatoryefficacy of methanolic extract of R. aquatica Lour. in in vivo models.Materials and methods: The qualitative phytochemical analysis and invitro antioxidant activity of theroots of methanolic extract of R. aquatica Lour. (MERA) was evaluated. The acute toxicity effect of MERAwas evaluated with two different doses (550, 2000 mg/kg body weight), were administrated orally toWistar rats. The rats were observed for sign and symptoms of toxicity and mortality for 14 days. Theparameters measured including relative organ weight, blood, biochemical and histopathological parameters of hepatic and renal toxicity. The anti-inflammatory effect of MERA was also evaluated incarrageenan and dextran-induced paw edema models.Results: The phytochemical evaluation of MERA shows the presence of secondary metabolites like alkaloids, flavonoids, phenolics and tannins, phytosterols, reducing sugars, proteins and terpenoids. Theresults of in-vitro antioxidant evaluation of MERA reveal its capability to scavenging free radical at alower concentration. The MERA did not show any visible signs of toxicity up to the dose of 2000 mg/kgbody weight. The results obtained from our carrageenan and dextran-induced paw edema model studyalso proved the anti-inflammatory effect of MERA in rat model.Conclusion: The result shows the potential of MERA as an anti-inflammatory drug to reduce the signs ofinflammation devoid of any toxic effect.© 2018 The Authors. Published by Elsevier B.V. on behalf of Institute of Transdisciplinary Health Sciencesand Technology and World Ayurveda Foundation. This is an open access article under the CC BY-NC-NDlicense (http//creativecommons.org/licenses/by-nc-nd/4.0/).