Antioxidant and immunomodulatory properties of Spilanthes oleracea with potential effect in chronic fatigue syndrome infirmity

ABSTRACT

Background: Chronic fatigue syndrome (CFS) holds a mystery for researchers due to its multifactorialnature; hence, its diagnosis is still based on symptoms and aetiology remains obscured. Number ofscientific evidences regarding the role of oxidative stress, immune dysfunction in CFS and alleviation ofsymptoms with the help of nutritional supplements guided us to study effect of ethanolic extract ofSpilanthes oleracea (SPE) in CFS.Objectives: Present study was designed to evaluate antioxidant, immunomodulatory properties of S.oleracea flower to ameliorate CFS infirmity in mice.Materials and method: In order to induce fatigue, experimental animals were stressed by chronic water eimmersion stress model. Meanwhile, parameters like immobility period and tail withdrawal latencywere assessed. On the 21st day, mice blood was collected and they were immediately sacrificed forbiochemical estimations.Results: Biochemical analysis results revealed that CFS elevates lipid peroxidation, nitrite level and diminishes the endogenous antioxidant enzyme like catalase level in stressed animal’s brain homogenate.Stressful condition developed muscle fatigue leading in alteration of lactate dehydrogenase level (LDH),Blood urea nitrogen (BUN) and Triglycerides (TG) levels. Concurrent and chronic treatment of SPE for 21days restored all these behavioural despairs and associated biochemical adaptation in mice in dosedependent manner.Conclusion: The outcome of this study indicates ability of SPE in amelioration of CFS by mitigating theoxidative stress and thus provide a powerful combat against CFS which may be due to its antioxidant andimmunomodulatory properties.© 2017 Transdisciplinary University, Bangalore and World Ayurveda Foundation. Publishing Services byElsevier B.V