Application of pulsed field gel electrophoresis for molecular characterization of vancomycin resistant Staphylococcus aureus isolates from hospitals
J Environ Biol
;
2020 Mar; 41(2): 202-206
Article
| IMSEAR
| ID: sea-214494
ABSTRACT
Aim:
The present study was conducted to characterize VRSA isolates on the basis of pulsed field gel electrophoresis (PFGE) and the presence of spa gene, recovered from different Doon Valley Hospitals.Methodology:
Six VRSA isolates were analyzed using PFGE and spa typing. spa gene coded Protein A was used as a genetic marker for the characterization of Staphylococcus aureus isolates. Dendrogram were constructed on the basis of unweighted pair group method with arithmetic means (UPGMA method) for clusters analyses.Results:
Dendrogram finally showed two major banding patterns at about 85% similarity designated as PFGE type A and PFGE type B exhibiting differences of 4-6 bands. The length of spa gene varied from 1200 to 1500 bp, showing variation in length. The most prevalent length was 1200bp.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Journal:
J Environ Biol
Year:
2020
Type:
Article
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