Molecular Method of Detection of Primary Endodontic Infection- An Ex Vivo Study.

ABSTRACT

The removal of microorganism and debris from the root canal system is the prerequisite for success of treatment. This can be achieved by thorough cleaning, shaping and disinfection of the root canal system. The aim of the present study is to investigate the presence of microorganism in primary endodontic infection in South Canara population using PCR technique.METHODSFifty patients with primary endodontic infection were selected for the study. Access cavity preparation was done followed by working length determination and first sample was collected by placing the paper point near the root apex for 1 min and immediately the samples were placed in Tris-EDTA buffer solution, stored at -200 C, followed by PCR analysis of the sample using specific primers for detection of microorganisms.RESULTSA total of 50 cases with primary endodontic infection were analysed for the presence of microorganism within the root canal system. Percentage analysis was done, and the positive results were obtained only for Porphyromonas endodontalis in 50 % of cases.CONCLUSIONSPorphyromonas endodontalis was the prevalent organism seen in primary endodontic infection in this particular geographic distribution.