Comparative Evaluation of Antimicrobial Efficacy of Mineral Trioxide Aggregate, Biodentine, EndoSequence against Bacterial Strains of Enterococcus faecalis- An In-Vitro Study.

ABSTRACT

Microorganisms are the common aetiological factors in initiation as well as perpetuation of periapical and pulpal diseases. Microbial leakage is a cardinal aetiology behind endodontic treatment failure. The success of root canal treatment is greatly influenced by antimicrobial potential of the dental material used in endodontic procedures which plays an important role in creating a hermetic seal to prevent egress of bacteria into root canal from periapical region. METHODSThe three cements MTA, Biodentine and EndoSequence were made into pellets to test their antimicrobial action on the growth of Enterococcus faecalis (ATCC 29212); method used was agar diffusion test. RESULTSAt 24-hour study interval, EndoSequence showed the greatest zone of inhibition of 10.6 mm against Enterococcus faecalis (ATCC 29212) compared to Biodentine with zone of inhibition of 5.5 mm and MTA with the zone of inhibition of 5 mm. EndoSequence showed highest antimicrobial efficacy at 36-hours and 72-hours study intervals by mean inhibition zone of 11 mm against Enterococcus faecalis compared to that of Biodentine (6 mm) and MTA (5.6 mm) respectively with a p value of less than 0.05 which is highly significant. The antimicrobial activity of EndoSequence was very strong compared to Biodentine and MTA against Enterococcus faecalis (ATCC 29212). CONCLUSIONSMicroorganisms play pivotal role in the initiation as well as perpetuation of periapical and pulpal diseases. Microbial leakage is a cardinal aetiology behind endodontic treatment failure. The success of the root canal treatment is greatly influenced by antimicrobial potential of the dental material used in endodontic procedures which plays an important role in creating a hermetic seal to prevent egress of bacteria into root canal from periapical region.