Antibacterial Activity of White Pepper Oleoresin Mediated Silver Nanoparticles against Oral Pathogens.

ABSTRACT

Plant mediated biological synthesis of silver nanoparticles is gaining importance due to its eco friendliness and simplicity. Different types of nanoparticles such as metal, metal oxide, metal sulphide and polymer nanoparticles are used in different types of applications in the fields of electronics, communications, electrical, mechanical, medical, dental etc. Aim of the present study was to evaluate the antibacterial activity of white pepper oleoresin mediated silver nanoparticles against oral pathogens. MethodsIn the present investigation, white pepper mediated synthesis of silver nanoparticles was carried out by the addition of silver nitrate with prepared white pepper oleoresin solution. White pepper oleoresin mediated silver nanoparticles were initially confirmed by colour changes and confirmed by UV- Visible spectroscopy. Anti-bacterial activity of the synthesised silver nanoparticles was carried out using agar well diffusion method. Different concentrations of silver nanoparticles were tested against different oral pathogens such as Staphylococcus aureus, Streptococcus mutans and Pseudomonas sp. ResultsThe solution containing white pepper oleoresin and silver nitrate solution showed a brown colour confirming the formation silver nanoparticles and peak at 350 nm revealed the silver nanoparticles surface plasmon resonance. The white pepper oleoresin mediated silver nanoparticles showed good antibacterial activity against all tested microorganisms and the maximum activity was found with Pseudomonas spp. followed by Staphylococcus aureus, and Streptococcus mutans. ConclusionsGreen synthesis of silver nanoparticles using white pepper oleoresin was of low cost and was convenient to carry out. Hence, white pepper oleoresin mediated silver nanoparticles may be used for the control of oral pathogens.