Oral Ulcer Healing after Treatment with Distilled Liquid Smoke of Coconut Shell on Diabetic Rats

ABSTRACT

Background: Distilled liquid smoke from coconut shell(Cocos nucifera L.) by pyrolysis process with final0 0temperature 400 C and then distilled in 120-150 C. Thecharacteristics of distilled liquid smoke are yellow3liquid, with acidity 2.39 and density 1.0643 g/cm . Themajor identified compounds arephenol (36.6%), 2-methoxyphenol (guaiacol) (25.2%), furfural (17.8%),2-methoxy-5-methylphenol (5.2%) and 4-ethyl-2-methoxyphenol (EMP) (3.5%) with 28 other minorconstituents by GCMS. Aim and Objectives: Distilledliquid smoke was examined to investigate its potentialtherapeutic to oral ulcer healing and diabetic conditionon the rat. Furthermore, the clinical oral ulcer healingwas evaluated based on ulcer size and diabetic wasevaluated based on Fasting Blood Glucose (FBG) andbody weight changes. Material and Methods: Diabeteswas induced by alloxan. Seventy-two hours afterinjection, the diabetic condition was confirmed withFBG of >200mg/dl, then the labial fornix anterior wasinjured to induce oral ulcer using round steel blade.Results: The clinical oral ulcer healing was improvedafter treatment with distillated liquid smoke comparedto benzydamine hydrochloride and aquadest sterile forthree days (p=0.005) and seven days (p=0.000).Treatment for seven days with distilled liquid smokeshowed significant improvement of the body masschanges compared to benzydamine hydrochloride(p=0.008) and aquadest sterile (p=0.002). There wasno improvement of FBG after treatment with distilledliquid smoke (p=0.152). Conclusion: Treatment withdistilled liquid smoke coconut shell can improved oralulcer healing and body weight changes, but not FBGchanges