Effect of Methanolic Extract of Securigera securidaca as Antioxidant and Antibacterial Activities

ABSTRACT

In the present work, the phytochemical screening, polyphenolic content, antibacterial activity and antioxidant activity of Securigera securidacaseeds in methanol were carried out. Phytochemical analysis of seeds showed the presence of alkaloids, flavonoids, saponins, terpenoids, steroids and glycosides. Total phenolic content was estimated by Folin Ciocalteau method and the result showed the highest phenolic content of 62.28 mg/g. Methanolic extract was screened for antibacterial activity by disc diffusion method and it found to be potent. The MIC of methanol extract identified by broth dilution method showed a MIC value of 0.25 mg/ml for both E. coliand Kl. Oxytoca, and also 0.5 mg/ml for both S. aureusand S. epidermis.The antioxidant effect of the seeds was tested by DPPH scavenging activity as in vitro assay. The extract had potent inhibitory activity (IC50) value of 0.057mg/ml. The finding experimental results showed that methanolic extract of Securigera securidacais important as a source of antibacterial activity and polyphenolic antioxidants.