The use of periovulatory human cervical mucus in human sperm capacitation and in-vitro fertilization of foreign eggs.
Bangladesh Med Res Counc Bull
;
1983 Jun; 9(1): 25-31
Article
in English
| IMSEAR
| ID: sea-224
ABSTRACT
The time requirement of a routine in-vitro fertilization experiment using human spermatozoa and zona-free hamster oocytes is more than one working day. We have used a simple process of simultaneous sperm washing and capacitation before challenging them against oocytes. Spermatozoa were recovered up to 90 minutes from the proximal end of a column of periovulatory human cervical mucus into Brinster, Whitten and Whittingham's (B.W.W.) medium. Without any further capacitation 50 microliters of these spermatozoal suspension, in the concentration of 2.5-3 X 10(6)/ml, fertilized 81% of a total of 100 eggs. A dramatic decrease in the fertilization rate (18%) was observed when these spermatozoa were further capacitated in-vitro for five hours before actual egg incubation for three hours. Both selection and accelerated capacitation may contribute to this result.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Oocytes
/
Ovulation
/
Ovum
/
Sperm Capacitation
/
Spermatozoa
/
Female
/
Humans
/
Male
/
Fertilization in Vitro
/
Cervix Mucus
Language:
English
Journal:
Bangladesh Med Res Counc Bull
Year:
1983
Type:
Article
Similar
MEDLINE
...
LILACS
LIS