Comparison of peripheral blood smear and automated cell counter in 100 cases of anemia

ABSTRACT

Background: Hemogram/ Complete Blood Count (CBC), performed on hematology analyzers, is one the most commonly ordered blood test in clinical practice especially in patients presenting with fever. The aim of the present study was to compare accuracy of RBC histograms shown by our 5 part cell counter to that of microscopy examination of blood film stain by field stain. Materials and methods: The present observational study was conducted during June 2020 - August 2020. Samples were run on 5 part hematology analyzer. Population of both anemic and healthy individuals in the age group 0-60 years were evaluated for the study. Total 100 patients in the age group of 0- 60 years were enrolled in this study. Hematological parameters were obtained by using 5 part automated hematology cell counter. The hemoglobin concentration and red blood cell (RBC) indices, encompassed mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) and erythrocyte count. Results: We studied 100 blood samples of patients. Among them 82 cases showed correlation between histogram to that of the peripheral blood smear examination.Conclusion: The result of the study shows that the automated hematology analyzer are appropriate for screening purposes because it increases the turnaround time and reduces the labor cost. But to diagnose and differentiate different types of anemia manual scan of peripheral smear is a method of choice.