Indigenous anti-hepatitis A virus IgM capture ELISA for the diagnosis of hepatitis A.
Article
in English
| IMSEAR
| ID: sea-22714
ABSTRACT
Anti-hepatitis A virus IgM capture ELISA was developed by using the reagents produced in the NIV laboratory. The major reagents of the assay were anti-human IgM antibody, hepatitis A virus (HAV) and anti-HAV IgG-horse radish peroxidase (HRP) conjugate. Of these, anti-human IgM antibodies were generated in rabbit against IgM secreted by human hybridoma clone(G3). HAV was derived from buffalo green money kidney cell line infected with HM-175 strain. Virus purified from the cell lysates was used for immunization of rabbits and guinea-pigs. There was very low anti-HAV response. A seropositive rhesus monkey was inoculated with monkey adapted strain of HAV to boost the anti-HAV antibody titre. Anti-HAV IgGs derived from hyperimmune sera of monkey and hepatitis A patient were conjugated with HRP. The preparations of conjugate--particularly human antibody--HRP conjugate yielded highly satisfactory results in anti-HAV capture ELISA. The assay appears to be specific, sensitive and quick and is useful in differentiating acute HAV infection from other acute infections caused by B, E and non-A non-B hepatitis viruses.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rabbits
/
Humans
/
Immunoglobulin M
/
Enzyme-Linked Immunosorbent Assay
/
Hepatitis Antibodies
/
Chlorocebus aethiops
/
Hepatovirus
/
Hepatitis A Antibodies
/
Guinea Pigs
/
Hepatitis A
Language:
English
Year:
1994
Type:
Article
Similar
MEDLINE
...
LILACS
LIS