Comparative study on determination of alpha-hydroxybutyrate dehydrogenase, lactate dehydrogenase isoenzyme LDH-1 by adsorption on DEAE Sephadex A-50 & electrophoresis.
Article
in English
| IMSEAR
| ID: sea-22856
ABSTRACT
Serum alpha-hydroxybutyrate dehydrogenase (HBDH) and lactate dehydrogenase isoenzyme (LDH-1) levels by two techniques viz., adsorption on DEAE Sephadex A-50 and by electrophoresis were estimated in healthy subjects and patients with testicular tumours. The correlation coefficient between HBDH and LDH-1 by adsorption technique was found to be r = 0.8. HBDH was found to have a value almost twice that of LDH-1 by adsorption and electrophoretic technique and the activity of LDH-1 obtained by two methods and of HBDH in normals and patients with testicular tumours could be converted using a factor of 0.557. The quantitative method used for determination of HBDH is reliable, accurate, simple and rapid and therefore has better value in a clinical setting than electrophoresis and adsorption techniques which are laborious and time consuming.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Testicular Neoplasms
/
Humans
/
Male
/
Chromatography, Ion Exchange
/
Electrophoresis
/
Hydroxybutyrate Dehydrogenase
/
Isoenzymes
/
L-Lactate Dehydrogenase
Language:
English
Year:
1992
Type:
Article
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