Analysis of the major surfactant-associated proteins in human lung.
Article
in English
| IMSEAR
| ID: sea-23699
ABSTRACT
The protein component of human pulmonary surfactant was analysed. A purified surfactant preparation, after delipidation, was subjected to gel filtration on Sephadex G-200. The proteins present in the surfactant were categorised by means of SDS-polyacrylamide gel electrophoresis into serum and non-serum components. Molecular masses determination showed the presence of three sub-groups with molecular masses of 60-68 kDa, 28-36 kDa and 10-18 kDa, respectively. Antiserum generated against 28-36 kDa protein strongly reacted with the purified surfactant and amniotic fluid, while it did not show any cross reactivity with other groups of proteins and serum in a double diffusion immunoprecipitation assay. We propose that this protein is the major non-serum surfactant-associated protein present in human lung surfactant.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Pulmonary Surfactants
/
Humans
/
Precipitin Tests
/
Blood Proteins
/
Chromatography, Gel
/
Adult
/
Electrophoresis, Polyacrylamide Gel
/
Immune Sera
/
Lung
/
Molecular Weight
Language:
English
Year:
1992
Type:
Article
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