Resolution of DL-Phenylglycine by Penicillin G acylase.
Hindustan Antibiot Bull
;
2005-2006; 47-48(): 41-4
Article
in English
| IMSEAR
| ID: sea-2375
ABSTRACT
The parameters for complete hydrolysis of L-phenyl acetyl phenylglycine (L-PAPG) using immobilized penicillin G acylase (IMEPGA) were investigated. IMEPGA exhibited maximum activity at pH 8.5 and 50 degrees C. The apparent Km value observed was 10 mM. Quantitative hydrolysis (>97%) of the L-PAPG was achieved within 45 min, at pH 7.8 and 37 degrees C, when 0.5% (w/v) of DL-PAPG was used and the concentration of IMEPGA was 133 IU/gm of DL-PAPG. The IMEPGA was used for 50 cycles.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Penicillin Amidase
/
Polymers
/
Temperature
/
Enzymes, Immobilized
/
Glycine
/
Hydrogen-Ion Concentration
Language:
English
Journal:
Hindustan Antibiot Bull
Year:
2005
Type:
Article
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