Applications of real-time PCR technology to mycobacterial research.
Article
in English
| IMSEAR
| ID: sea-23894
ABSTRACT
Real-time polymerase chain reaction (PCR) is being used worldwide for various research purposes. In this review we discuss the principles of real-time PCR, different methodologies and chemistries available and their applications in mycobacterial research. This technology allows for the direct detection of PCR product during the exponential phase of the reaction. Being a very powerful, accurate, rapid and sensitive method, this technique holds immense promise for detection of mycobacteria, differentiation of mycobacterial species, quantification of mycobacterial load and detection of drug resistance in mycobacterial infection.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Species Specificity
/
Humans
/
Colony Count, Microbial
/
Polymerase Chain Reaction
/
Drug Resistance, Bacterial
/
Mycobacterium
/
Mycobacterium Infections
/
Mycobacterium tuberculosis
Language:
English
Year:
2006
Type:
Article
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