An ELISA for quantitation of tetanus toxin.
Article
in English
| IMSEAR
| ID: sea-25018
ABSTRACT
A sandwich ELISA for quantitation of tetanus toxin was devised using commercially available horse antitetanus antiserum and its HRP-conjugate. With commercial toxoid as a standard the assay had a lower sensitivity of 100 ng/ml and was linear up to 100 micrograms/ml. In terms of Lf equivalents the ELISA assay was linear from 0.02 to 20 Lf/ml. The Lf activity in 20 preparations of tetanus toxin as determined by ELISA was comparable to the activity measured by conventional flocculation tests (r = 0.987).
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Tetanus Toxin
/
Enzyme-Linked Immunosorbent Assay
/
Predictive Value of Tests
/
Flocculation
Language:
English
Year:
1990
Type:
Article
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