Detection of glutamate dehydrogenase enzyme activity in Plasmodium falciparum infection.
Article
in English
| IMSEAR
| ID: sea-25091
ABSTRACT
We describe the separation of an active glutamate dehydrogenase [GDH (NADP+)] enzyme from the plasma of patients with P. falciparum infection using columns of sepharose anti-GDH (NADP+) of Proteus spp. The activity of this enzyme was also detected in P. falciparum culture supernatant. The parasitic origin of this enzyme was suggested by western blot analysis using anti-P. falciparum culture supernatant and anti-whole parasite antibodies. The differential inhibition of the P. falciparum GDH (NADP+) indicates that some epitopes recognised by the antibodies in both preparations may be different. The determination of P. falciparum GDH (NADP+) activity could be developed into a specific technique for the diagnosis of falciparum malaria.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plasmodium falciparum
/
Humans
/
Malaria, Falciparum
/
Glutamate Dehydrogenase
/
Animals
Type of study:
Diagnostic study
Language:
English
Year:
1999
Type:
Article
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