Evaluation of product analogues for purification of penicillinase by affinity chromatography.

Kumar, K K; Deshpande, B S; Ambedkar, S S; Shewale, J G

Kumar, K K; Deshpande, B S; Ambedkar, S S; Shewale, J G.

Hindustan Antibiot Bull ; 1996 Feb-Nov; 38(1-4): 37-40

Article in English | IMSEAR | ID: sea-2528

ABSTRACT

ABSTRACT

Among different matrices prepared, ampicilloic acid-polymer matrix offered 86.7% adsorption, 95% elution and 82.4% overall recovery of penicillinase. The structure of both the side chain and penicilloic or cephalosporoic acid moieties contribute to the affinity interactions.

Subject(s)

Adsorption; Bacillus cereus/enzymology; Cephalosporins/chemistry; Chromatography, Affinity/methods; Fermentation; Ligands; Penicillanic Acid/analogs & derivatives; Penicillinase/analysis

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Penicillinase / Bacillus cereus / Cephalosporins / Chromatography, Affinity / Penicillanic Acid / Adsorption / Fermentation / Ligands Language: English Journal: Hindustan Antibiot Bull Year: 1996 Type: Article

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