Vaccine potential of 56-66 kDa protease secreted by Entamoeba histolytica.
Article
in English
| IMSEAR
| ID: sea-25977
ABSTRACT
Excretory/secretory (ES) antigens and sub-cellular fractions of E. histolytica (HM1IMSS strain) were tested for the presence of common proteases using substrate gel electrophoresis. We obtained two E. histolytica proteases (56-66 kDa and 29 kDa) from ES material, soluble components and plasma membrane. Protease 56-66 kDa from ES antigen was selected for immunizing hamsters because it gave a consistent broad band. We observed 62.5 per cent protection in immunized animals, compared to 0 per cent in unimmunized controls. Although all vaccinated golden hamsters showed high antibody response, there was no correlation between antibody titres and protection. 56-66 kDa ES protease could thus prevent disease and could be a candidate molecular vaccine against amoebiasis.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Endopeptidases
/
Antibodies, Protozoan
/
Protozoan Vaccines
/
Cricetinae
/
Mesocricetus
/
Immunization
/
Amebiasis
/
Amoeba
/
Animals
/
Molecular Weight
Language:
English
Year:
1999
Type:
Article
Similar
MEDLINE
...
LILACS
LIS