Isolation and purification of penicillin G acylase obtained from Escherichia coli (NCIM-2400) and immobilisation on Eupergit C for the production of 6 amino penicillanic acid.
Hindustan Antibiot Bull
;
1997 Feb-Nov; 39(1-4): 1-10
Article
in English
| IMSEAR
| ID: sea-2619
ABSTRACT
Penicillin G-Acylase is produced by submerged cultivation of E. Coli (NCIM-2400) and extracted from the harvested fermented broth, purified (affinity chromatography) and immobilised on Eupergit C (Synthetic polymer in bead form). The immobilised penicillin G acylase properties are studied and compared with soluble penicillin G-acylase. The control parameters for conversion of penicillin G-K to 6 APA are optimised [e.g. substrate (Pen G-K) concentration ratio to immobilised penicillin G-acylase, temperature, pH etc.] in a stirred tank reactor. Our findings suggest that immobilised penicillin G-acylase can be used commercially and the productivity of 1 kg. of immobilised enzyme is around 400 kg of 6 APA under given desired stipulated conditions.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Penicillin Amidase
/
Polymers
/
Penicillanic Acid
/
Enzymes, Immobilized
/
Escherichia coli
/
Hydrogen-Ion Concentration
Language:
English
Journal:
Hindustan Antibiot Bull
Year:
1997
Type:
Article
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