Characterization of mouse fibroblast (NIH3T3) and fibrosarcoma cell lines (WEHI-164 and MFS 8) using PMRS.

Jayashree, B; Visalakshi, V; Rajalakshmi, K R; Sukumaran, M S; Moni, M S; Rajkumar, T; Deshmukh, S

Jayashree, B; Visalakshi, V; Rajalakshmi, K R; Sukumaran, M S; Moni, M S; Rajkumar, T; Deshmukh, S.

Indian J Biochem Biophys ; 1998 Apr; 35(2): 108-14

Article in English | IMSEAR | ID: sea-26252

ABSTRACT

ABSTRACT

Proton magnetic resonance Spectroscopy (PMRS) has been used to study the differences between immortalized fibroblasts and fibrosarcoma cells of different grade. One and two dimensional purged correlation spectroscopy (PCOSY) have been used to assess intact viable fibroblast and fibrosarcoma cells, and differences in the triglyceride, cellular metabolite, and cell surface fucosylation patterns between the three cell lines have been observed. The clinical implication of this study is the potential use of PMRS as an adjunct to conventional histopathology.

Subject(s)

3T3 Cells/cytology; Amino Acids/metabolism; Animals; Cell Cycle; Cell Line, Transformed; Cell Membrane/metabolism; Cell Survival; Fibrosarcoma/metabolism; Fucose/analysis; Magnetic Resonance Spectroscopy/methods; Mice; Phospholipids/metabolism; Triglycerides/metabolism; Tumor Cells, Cultured

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Phospholipids / Triglycerides / Cell Line, Transformed / Tumor Cells, Cultured / Magnetic Resonance Spectroscopy / Cell Cycle / Cell Membrane / Cell Survival / 3T3 Cells / Fibrosarcoma Language: English Journal: Indian J Biochem Biophys Year: 1998 Type: Article

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