Further studies on proteinases and alpha 2-macroglobulin activity in diabetic plasma.
Indian J Biochem Biophys
;
1992 Apr; 29(2): 189-91
Article
in English
| IMSEAR
| ID: sea-26254
ABSTRACT
Loss of chymotrypsin binding capacity of alpha 2-macroglobulin in diabetic plasma on in vitro incubation, could be partially prevented by phenylmethyl sulphonyl fluoride and pepstatin A. Prior ten-fold dilution of plasma with 0.02 M phosphate buffer (pH 7.0) completely arrested the process. The phenomenon could not be reactivated by Ca2+, lecithin or bovine serum albumin. Diabetic plasma, like normal plasma, exhibited maximal hydrolytic activities on H-D-Pro-Phe-Arg-p-nitroanilide, H-D-Val-Leu-Arg-p-nitroanilide and H-D-Ile-Pro-Arg-p-nitroanilide. The hydrolytic activities were not significantly diminished on incubation of plasma at 37 degrees C for 12 hr, unlike alpha 2-macroglobulin activity. On gel chromatography on Sephadex G-200, part of the proteolytic activity in diabetic plasma coeluted with alpha 2-macroglobulin in the VO region. A second activity peak (absent in normal plasma) was eluted with a Ve/V0 value of 1.40. Possible role of free proteinases in diabetic plasma in the inactivation of alpha 2-macroglobulin is discussed.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Oligopeptides
/
Endopeptidases
/
Protease Inhibitors
/
Substrate Specificity
/
Humans
/
Alpha-Macroglobulins
/
Molecular Sequence Data
/
Chymotrypsin
/
Amino Acid Sequence
/
Diabetes Mellitus
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1992
Type:
Article
Similar
MEDLINE
...
LILACS
LIS