Construction, purification and characterization of a recombinant baculovirus containing the gene for alpha subunit of human chorionic gonadotropin.
Indian J Biochem Biophys
;
1991 Aug; 28(4): 237-42
Article
in English
| IMSEAR
| ID: sea-26280
ABSTRACT
A cDNA encoding the alpha subunit of human chorionic gonadotropin, a placental glycoprotein hormone, was cloned downstream to the viral polyhedrin gene promoter of Autographa california nuclear polyhedrosis virus and the recombinant transfer vector was used to co-transfect Spodoptera frugiperda cells growing in culture. Recombinant baculovirus carrying the alpha hCG gene was detected and isolated after dot hybridization using supernatant from co-transfected cells. Recombinant vAc alpha hCG having a replacement of the viral polyhedrin gene, which is hyper-transcribed very late in the infection cycle, with the alpha hCG cDNA was purified after a single round of plaque purification. Insect cell culture infected with vAc alpha hCG, secreted high levels of hCG which was biologically active.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
DNA
/
Glycoprotein Hormones, alpha Subunit
/
Restriction Mapping
/
Baculoviridae
/
Cloning, Molecular
/
Genetic Vectors
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1991
Type:
Article
Similar
MEDLINE
...
LILACS
LIS