Role of positive charge of lysine residue on ribosome-inactivating property of gelonin.
Indian J Biochem Biophys
;
2001 Oct; 38(5): 309-12
Article
in English
| IMSEAR
| ID: sea-26321
ABSTRACT
The report that gelonin cross-linked with monoclonal antibodies with the use of 2-iminothiolane (2-IT) exhibited higher cytotoxicity than the conjugates prepared with the use of N-succinimidyl-3-(2-pyridylthio) propionate (SPDP) alone, has prompted us to investigate the effect of epsilon-NH2 group modification with 2-IT on the ribosome-inactivating property (RIP) of gelonin. The purified gelonin was modified with 2-IT at a different molar ratio and their effects on immunoreactivity and ribosome-inactivating property were compared with those of N-succinimidyl 6-[3-(2-pyridyldithio) propionamido] hexanoate (long chain-SPDP) and SPDP modified gelonin derivatives. Modification of single amino group with 2-IT results in about 25-50% inhibition of immunoreactivity and 60-70% loss of protein synthesis inhibition activity. Modification of 2-3 amino groups further hampers both immunoreactivity and protein synthesis inhibition property of gelonin. Both the long chain-SPDP with SPDP modifications showed more pronounced effects on immunoreactivity and RIP activity as compared to the similar ratio of 2-IT modification(s). It may, therefore, be concluded that the positive charge plays an important role in the immunological as well as the protein synthesis inhibitory effect of gelonin.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plant Proteins
/
Ribosomes
/
Succinimides
/
Protein Synthesis Inhibitors
/
Cell-Free System
/
Cross-Linking Reagents
/
Deoxyribonucleases
/
Ribosome Inactivating Proteins, Type 1
/
Imidoesters
/
Lysine
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2001
Type:
Article
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