Biologically active polymer supports based on cellulosic derivatives: synthesis and kinetic study.
Indian J Biochem Biophys
;
1989 Apr; 26(2): 80-3
Article
in English
| IMSEAR
| ID: sea-26538
ABSTRACT
Bioactive cellulose derivatives have been synthesised by coupling enzymes/antibiotics on carboxymethyl cellulose acid chloride and cellulose carbonate. The effect of pH and temperature on the enzymatic activity of amyloglucosidase immobilised on cellulose carbonate was studied. Michaelis-Menten kinetics have been obeyed to the first degree of approximation despite the restricted mobility of the attached enzyme on the polymer support. Lineweaver-Burk plots for the amyloglucosidase immobilized on carboxymethyl cellulose acid chloride at ambient pH with cellulose carbonate at pH 8 have also been plotted. The Michaelis-Menten constant for the immobilized amyloglucosidase on cellulose carbonate at pH 8 was 9.1 mM, and the activation energy for starch hydrolysis was 21.8 kcals/mole.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Polymers
/
Kinetics
/
Cellulose
/
Chemistry
/
Chemical Phenomena
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1989
Type:
Article
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