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Biologically active polymer supports based on cellulosic derivatives: synthesis and kinetic study.
Indian J Biochem Biophys ; 1989 Apr; 26(2): 80-3
Article in English | IMSEAR | ID: sea-26538
ABSTRACT
Bioactive cellulose derivatives have been synthesised by coupling enzymes/antibiotics on carboxymethyl cellulose acid chloride and cellulose carbonate. The effect of pH and temperature on the enzymatic activity of amyloglucosidase immobilised on cellulose carbonate was studied. Michaelis-Menten kinetics have been obeyed to the first degree of approximation despite the restricted mobility of the attached enzyme on the polymer support. Lineweaver-Burk plots for the amyloglucosidase immobilized on carboxymethyl cellulose acid chloride at ambient pH with cellulose carbonate at pH 8 have also been plotted. The Michaelis-Menten constant for the immobilized amyloglucosidase on cellulose carbonate at pH 8 was 9.1 mM, and the activation energy for starch hydrolysis was 21.8 kcals/mole.
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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Polymers / Kinetics / Cellulose / Chemistry / Chemical Phenomena Language: English Journal: Indian J Biochem Biophys Year: 1989 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Polymers / Kinetics / Cellulose / Chemistry / Chemical Phenomena Language: English Journal: Indian J Biochem Biophys Year: 1989 Type: Article