Oral and parenteral immunization with synthetic retro-inverso peptides induce antibodies that cross-react with native peptides and parent antigens.
Indian J Biochem Biophys
;
2007 Jun; 44(3): 140-4
Article
in English
| IMSEAR
| ID: sea-26545
ABSTRACT
The objective of this study was to determine whether certain retro-inverso peptides have the potential to act as synthetic vaccines in mice, when immunized by injection or orally. Immunization of mice parenterally with conjugates of three such retro-inverso peptides and orally with the unconjugated peptides elicited generally high titres of anti-peptide antibodies. Antibodies against the same three peptides cross-reacted by binding strongly in ELISA to the native peptides and vice versa, regardless of the mode of immunization. Antibodies against a retro-inverso diphtheria peptide also reacted strongly with diphtheria toxin. Seven of 8 mice, immunized by injection of the conjugate of a retro-inverso derivative of robustoxin [a lethal spider (Atrax robustus) venom toxin] were protected from challenge involving injection with twice the minimum lethal dose of A. robustus venom containing the toxin.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Peptides
/
Plasmodium falciparum
/
Serine
/
Spider Venoms
/
Enzyme-Linked Immunosorbent Assay
/
Vaccines, Synthetic
/
Adjuvants, Immunologic
/
Administration, Oral
/
Immunization
/
Vaccination
Type of study:
Prognostic study
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2007
Type:
Article
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