Rat liver chromatin digested in situ with endogeneous Mg(2+)-dependent deoxyribonuclease exhibits two distinct DNA repeats.
Indian J Biochem Biophys
;
1992 Feb; 29(1): 42-8
Article
in English
| IMSEAR
| ID: sea-26625
ABSTRACT
Incubation of rat liver nuclei in the presence of 1.0-5.0 mM Mg2+ at 37 degrees C releases oligonucleosomes containing at least two distinct chromatin-DNA repeat elements. The 'short' repeat is derived from the dimer to pentamer series, while the 'long' repeat is found in the monomer and hexamer to decanucleosomes. Both repeat lengths decrease during enzymatic hydrolysis but in 5.0 mM Mg2+, which is optimal concentration, the 'long' repeat is degraded faster.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rats
/
DNA
/
Chromatin
/
Repetitive Sequences, Nucleic Acid
/
Endodeoxyribonucleases
/
Liver
/
Animals
/
Magnesium
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1992
Type:
Article
Similar
MEDLINE
...
LILACS
LIS