Plausible involvement of extralysosomal proteinase in derangement of leucocytic Ca2+/Mg2+ ATPase in diabetes.

ABSTRACT

Translocation of calcium activated neutral proteinase from cytosol to plasma membrane, concurrently with decrease in the activity of membrane bound Ca2+/Mg2+ ATPase has been detected in diabetic polymorphonuclear leucocytes. Plausible involvement of the extralysosomal proteinase in the derangement of the Ca2+/Mg2+ ATPase is indicated by non restoration of the enzyme activity on treatment with activators such as trypsin or calmodulin and enhanced membrane translocation of the proteinase observed with concomitant decrease in the activity of Ca2+/Mg2+ ATPase in normal neutrophils on insult with diabetic serum factor.