Spectrophotometric and spectrofluorometric titrations of teichoic acid.
Indian J Biochem Biophys
;
1989 Oct; 26(5): 311-4
Article
in English
| IMSEAR
| ID: sea-26806
ABSTRACT
Spectrophotometric and spectrofluorometric titrations of two strongly aggregating dyes, 1,9-dimethylmethylene blue (DMMB) and acridine orange (AO), by three anionic biopolymers, chondroitin sulphate A, DNA and teichoic acid (TA), have been described. Though the three polymers differ in their dye binding efficiencies and TA is a weak chromotrope, the equivalent weights of the polymers can be estimated accurately by these methods using the two dyes. Results show DMMB to be the preferred dye for spectrophotometric titration. The titrations can be used to estimate the equivalent weights of anionic polymers, and also for the quantitative estimation of such polymers of known equivalent weights.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Spectrometry, Fluorescence
/
Spectrophotometry
/
Teichoic Acids
/
Acridine Orange
/
Methylene Blue
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1989
Type:
Article
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