Regulation of MAL1+ gene expression encoding maltase in Schizosaccharomyces pombe by added inositol.
Indian J Biochem Biophys
;
2006 Jun; 43(3): 143-7
Article
in English
| IMSEAR
| ID: sea-26850
ABSTRACT
In this study, the effects of inositol addition on maltase activity and expression of MAL1+ gene encoding maltase in Schizosaccharomyces pombe were investigated. The maximum specific maltase activity was observed, when the concentration of inositol reached 6.0 microg/ml in the synthetic medium containing 2.0% glucose. At 1.0 microg/ml inositol concentration, the maltase activity continuously decreased, as initial glucose concentration was higher than 0.1%. mRNA encoding maltase and phosphatidylinositol (PI) content were higher in the cells grown in the synthetic medium with 6.0 microg/ml of inositol and 2.0% glucose than those with 1.0 microg/ml of inositol. These results demonstrated that higher inositol concentration in the synthetic medium could derepress MAL1+ gene expression in S. pombe and PI might be involved in derepression of MAL1+ gene expression in S. pombe probably by PI-type signalling pathway.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phospholipids
/
Schizosaccharomyces
/
Gene Expression Regulation, Fungal
/
Alpha-Glucosidases
/
Genes, Fungal
/
Glucose
/
Inositol
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2006
Type:
Article
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