Determination of plasma oxalate with chloride ion insensitive oxalate oxidase.
Indian J Biochem Biophys
;
1998 Apr; 35(2): 120-2
Article
in English
| IMSEAR
| ID: sea-26916
ABSTRACT
A simple colorimetric method has been developed for determination of oxalate in plasma using a C1-insensitive oxalate oxidase purified from grain sorghum leaves. The ultrafiltered plasma collected in 3.5 N HC1 was pretreated with NaNO2 to avoid possible interference by ascorbate. The minimum detection limit of the method is 0.225 mg/l. The % recovery of the added oxalate was 91.5 +/- 5.0 (mean +/- S.D., n = 15). The coefficient of variation within and between batch were < 3 and < 5 respectively. The mean plasma oxalate concentration in healthy subjects was 0.29 mg/l. The method has the advantage over other enzymic methods that it doesn't require the removal of C1- prior to oxalate assay.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Oxalates
/
Oxidoreductases
/
Reference Values
/
Spectrophotometry
/
Humans
/
Male
/
Kinetics
/
Edible Grain
/
Chlorides
/
Sensitivity and Specificity
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1998
Type:
Article
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