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Superhelical density of goat mitochondrial DNA: fluorimetric studies.
Indian J Biochem Biophys ; 1994 Feb; 31(1): 77-9
Article in English | IMSEAR | ID: sea-26978
ABSTRACT
EtBr binding to DNA has been used to determine the superhelical density of Goat mtDNA. The differential binding of closed circular and nicked circular DNA is estimated by simple fluorescence measurements, which is a function of state of DNA supercoiling. The method is simple and quick over the cesium chloride density gradient centrifugation studies.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: DNA, Mitochondrial / DNA, Superhelical / Goats / Fluorometry / Animals Language: English Journal: Indian J Biochem Biophys Year: 1994 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: DNA, Mitochondrial / DNA, Superhelical / Goats / Fluorometry / Animals Language: English Journal: Indian J Biochem Biophys Year: 1994 Type: Article