Identification and estimation of endogenous digoxin in biological fluids and tissues by TLC and HPLC.
Indian J Biochem Biophys
;
1998 Oct; 35(5): 308-12
Article
in English
| IMSEAR
| ID: sea-27024
ABSTRACT
A procedure for estimation of digoxin in biological samples after adding a known quantity of digoxin followed by extraction, separation by TLC and HPLC is described. The identity of digoxin thus extracted from rat brain has been established by reaction with digoxin antibody and by its inhibition of Na(+)-K+ ATPase activity. The method could be a better substitute to the routine radioimmunoassay as interfering substances are removed by TLC and HPLC.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rats
/
Body Fluids
/
Brain Chemistry
/
Male
/
Chromatography, High Pressure Liquid
/
Chromatography, Thin Layer
/
Rats, Wistar
/
Digoxin
/
Animals
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1998
Type:
Article
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