Inhibition of rabbit intestinal brush border sucrase by indolin 2,3-dione (isatin) at pH 5.0.
Indian J Biochem Biophys
;
1994 Jun; 31(3): 191-4
Article
in English
| IMSEAR
| ID: sea-27425
ABSTRACT
Isatin (10 mM) inhibited the activity of rabbit brush border sucrase by 60% at pH 5.0 but it had no effect on enzyme activity around neutral pH. Isatin inhibition of sucrase was unaffected by Na+ ions but K+ and Cs+ ions reduced enzyme inhibition, partially. Kinetic analysis revealed that sucrase inhibition by isatin at acidic pH was non-competitive with Ki of the order 6.5-7.8 mM. Isatin together with 4 mM harmaline or iodoacetate (3 mM) or dithionitrobenzene (2 mM) yielded 80-85% inhibition of the enzyme. These observations suggest that inhibitory sites for isatin, harmaline and -SH group reacting agents are distinct in rabbit brush border sucrase.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rabbits
/
Sucrase
/
Hydrogen-Ion Concentration
/
Intestines
/
Isatin
/
Animals
/
Microvilli
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1994
Type:
Article
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