Comparative analysis of surface components of adult, micro-filariae and infective larvae of Brugia malayi.
Indian J Biochem Biophys
;
1989 Feb; 26(1): 52-5
Article
in English
| IMSEAR
| ID: sea-27489
ABSTRACT
A comparative analysis of surface proteins of adult, microfilariae and infective larvae of Brugia malayi, the human filarial parasite, has been carried out using IODOGEN (1,3,4,6-tetrachloro-3,alpha 6 alpha-diphenyl-glycoluril) and lactoperoxidase methods. SDS-polyacrylamide gel electrophoretic and autoradiographic analyses revealed the presence of 9 proteins (15-200 kDa) in adults, while microfilariae and infective larvae showed 8 and 6 proteins (15-120 kDa), respectively. The pattern of proteins radiolabelled by IODOGEN method was very similar to that of proteins labelled by the lactoperoxidase method. Since these proteins are released by the protease treatment of whole parasites, they are likely to be present on the surface of the parasite.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Urea
/
Brugia
/
Iodine Radioisotopes
/
Lactoperoxidase
/
Larva
/
Animals
/
Membrane Proteins
/
Microfilariae
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1989
Type:
Article
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