Molecular cloning of human satellite DNA sequences and their use in detecting DNA polymorphism.
Indian J Biochem Biophys
;
1990 Oct; 27(5): 275-9
Article
in English
| IMSEAR
| ID: sea-27713
ABSTRACT
A approximately 400 bp HaeIII human genomic satellite DNA band was cloned into pUC18 to construct a partial library. A fragment of bacteriophage M13 containing a sequence homologous to the human minisatellite core was cloned in pUC18 and was used as a probe to isolate a approximately 350 bp human satellite clone (pTRF5.6) from the partial library. Other clones from this library showed a wide variation in terms of size and hybridization to the pTRF5.6 clone. Human DNA from different individuals was digested with restriction enzymes, Southern transferred and probed with TRF5.6. Individual-specific complex pattern of DNA bands was produced. TRF5.6, therefore, could be useful as a probe for detecting genetic polymorphism.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Polymorphism, Genetic
/
Humans
/
DNA, Satellite
/
Molecular Sequence Data
/
Base Sequence
/
Cloning, Molecular
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1990
Type:
Article
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