Effect of a fatty acid moiety of phospholipid and ceramide on purified GalT-3 (UDP-Gal:GM2 beta 1-3 galactosyltransferase) activity from embryonic chicken brain.
Indian J Biochem Biophys
;
1990 Dec; 27(6): 379-85
Article
in English
| IMSEAR
| ID: sea-27727
ABSTRACT
Galactosyltransferase, GalT-3 (UDP-GalGM2 beta 1-3 galactosyltransferase) has been characterized and solubilized from 19-day-old embryonic chicken brain, and purified to over 2000-fold using mixed-modal chromatography on a omega-aminohexyl Sepharose column and affinity chromatography on a UDP-hexanolamine Sepharose column. The activity of purified GalT-3 was modulated by phospholipids in vitro with stimulation observed specifically with dipalmitoyl phosphatidylethanolamine (PE). All natural phospholipids tested (PE, PC and PI) inhibited GalT-3 activity. Enzyme activity was affected by the structure of the phospholipid vesicle. It was stabilized by the hexagonal (dipalmitoyl PE) structure and inhibited by the bilayer (dielaidoyl PE) structure. The long-chain fatty acid moiety of the glycosphingolipid substrate, GM2, was found to be necessary for optimum enzyme activity. In the absence of fatty acid, the modified substrates, lyso-GM2 and acetyl-GM2, had a 10-fold increased Km and a 4-8 fold decreased Vmax compared to the normal substrate. We postulate that GalT-3 belongs to a group of glycosyltransferases having recognition for both the carbohydrate as well as the hydrophobic domains (HY-CARS) of their substrates and that the fatty acid moiety of either the substrate (GM2) or a heterotropic effector (phospholipid) plays an important role in regulating the activity of this enzyme.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phospholipids
/
Kinetics
/
Centrifugation, Density Gradient
/
Ceramides
/
Chick Embryo
/
Chromatography, Affinity
/
Chromatography, Agarose
/
Ganglioside Galactosyltransferase
/
Fatty Acids
/
Galactosyltransferases
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1990
Type:
Article
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