Metabolism of arachidonic acid in sheep uterus: in vitro studies.
Indian J Biochem Biophys
;
2007 Aug; 44(4): 216-22
Article
in English
| IMSEAR
| ID: sea-27743
ABSTRACT
Arachidonic acid (AA) metabolism in the non-pregnant sheep uterus was studied in vitro using conventional chromatographic and HPLC techniques. High expression of both lipoxygenase (LOX) as well as cyclooxygenase (COX) enzymes and their activities was found in the uterine tissues. On incubation of uterine enymes with AA, the LOX products formed were identified as 5-hydroperoxyeicosatetraenoic acid (5-HPETE), 12- and 15-hydroxyeicosatetraenoic acids (12- and 15-HETEs), based on their separation on TLC and HPLC. By employing differential salt precipitation techniques, the LOXs generating products 5-HPETE (5-LOX), 12-HETE and 15-HETE (12- and 15-dual LOX) were isolated. Based on their analysis on TLC, the COX products formed were identified as prostaglandins - PGF2alpha and prostacyclin derivative 6-keto PGF1alpha. The study forms the first report on the comprehensive analysis on the metabolism of AA in sheep uterus in vitro via the LOX and COX pathways.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Uterus
/
Female
/
Sheep
/
6-Ketoprostaglandin F1 alpha
/
Dinoprost
/
Hydroxyeicosatetraenoic Acids
/
Leukotrienes
/
Chromatography, High Pressure Liquid
/
Chromatography, Thin Layer
/
Arachidonic Acid
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2007
Type:
Article
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