Cloning, expression and purification of the DNA binding domain of RFX protein.
Indian J Biochem Biophys
;
1999 Dec; 36(6): 429-32
Article
in English
| IMSEAR
| ID: sea-27899
ABSTRACT
The RFX DNA binding domain (DBD) is a novel highly conserved motif belonging to a large number of dimer DNA binding proteins which have diverse regulatory functions in eukaryotic organisms. To characterize this novel motif, a 78mer polypeptide corresponding to the DBD of human hRFX (hrfX1/DBD), a prototypical member of the RFX family has been cloned and overproduced in Escherichia coli. A purification procedure using cation exchange chromatography has also been developed.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Transcription Factors
/
Humans
/
DNA
/
Molecular Sequence Data
/
Base Sequence
/
Polymerase Chain Reaction
/
Amino Acid Sequence
/
Cloning, Molecular
/
DNA-Binding Proteins
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1999
Type:
Article
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