Cloning and characterization of mycobacteriophage I3 promoters.
Indian J Biochem Biophys
;
1995 Dec; 32(6): 361-7
Article
in English
| IMSEAR
| ID: sea-27901
ABSTRACT
Restriction fragments of mycobacteriophage I3 DNA capable of initiating transcription have been cloned into a promoter selection vector of Escherichia coli, and selected on the basis of development of resistance to chloramphenicol. The growth pattern of these 'promoter clones' on a concentration gradient of chloramphenicol and the biochemical assays of the chloramphenicol acetyl transferase have permitted the assessment of their relative promoter strengths. DNA sequence analysis revealed significant homology of these promoters to the -35 regions of the mycobacterial--and E. coli promoter consensus, but less so the -10 region. Based on the sequence of phage I3 promoters identified here and the reported sequences of mycobacterial promoters, a promoter consensus for mycobacteria has been generated.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Molecular Sequence Data
/
Base Sequence
/
Promoter Regions, Genetic
/
Cloning, Molecular
/
Mycobacteriophages
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1995
Type:
Article
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