A minor 9.8 kb rDNA unit of rice variety IR-20.
Indian J Biochem Biophys
;
1989 Oct; 26(5): 289-92
Article
in English
| IMSEAR
| ID: sea-28024
ABSTRACT
A clone bearing a 9.8 kb EcoRI fragment of rice DNA containing the genes for the rRNAs and the intergenic spacer was identified by screening a rice genomic library in lambda Charon 4 phage with rRNAs. The 9.8 kb EcoRIDNA fragment was found to be a minor rDNA unit of rice variety IR-20. The rRNA genes and the intergenic spacer were mapped by hybridization and nucleotide sequence analyses. The DNAs in the intergenic spacer of the minor rDNA unit of 9.8 kb and the major rDNA unit of 8.9 kb cross-hybridized showing that those regions are homologous.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Oryza
/
DNA, Ribosomal
/
Molecular Sequence Data
/
Base Sequence
/
Repetitive Sequences, Nucleic Acid
/
Restriction Mapping
/
Regulatory Sequences, Nucleic Acid
/
Cloning, Molecular
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1989
Type:
Article
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