Cloning and sequencing of an apparently recombinant promoter for napin gene from Brassica campestris genomic library and its evolutionary significance.
Indian J Biochem Biophys
;
1999 Aug; 36(4): 221-6
Article
in English
| IMSEAR
| ID: sea-28112
ABSTRACT
From a genomic library of Brassica campestris (brown sarson cv. B54), we have cloned and sequenced about 2 kb of upstream regulatory region from one of the 2S albumin-coding gene family. The sequence has several seed-specific promoter motifs. A sequence alignment of the 5' flanking regions of the available Brassica 2S storage protein genes showed that our sequence is a double crossover recombinant product of the two members of the napin gene family. A possible explanation of this fact is that Brassica species evolved through gene duplication and recombination from a common ancestor with fewer number of chromosomes and genes.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plant Proteins
/
Recombination, Genetic
/
Brassica
/
Molecular Sequence Data
/
Base Sequence
/
Sequence Homology, Nucleic Acid
/
Promoter Regions, Genetic
/
Cloning, Molecular
/
DNA, Complementary
/
Evolution, Molecular
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1999
Type:
Article
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