Studies on the surface properties of human lymphocytes by photon correlation spectroscopy technique.

ABSTRACT

Electrokinetic behaviour of human lymphocytes was studied by photon correlation spectroscopy technique on laser IR-spectrometer. The electrophoretic mobilities (EPMs) were measured for peripheral blood lymphocytes (PBL) and CEM-C12 T cell line in 11 electrolyte at 22 degrees C. Plots of mobility vs ionic strength in the range 0.001-0.1 M were compared with theoretical curves calculated from (i) the Smoluchowsky formula, (ii) the simplified form of the Dukhin-Deryaguin equation which takes into account the fact that the mobility decreases due to the relaxation effect and (iii) the equation suggested by Donath and Pastushenko, which takes into account the influence of cell glycoprotein layer (GPL) on the EPM values. It has been found that the first two equations describe the experimental data with the assumption that surface charge density (sigma) decreases and width of the hydrodynamically immobile layer (L) increases with decreasing ionic strength; the relaxation effect turns out to be insignificant for the cell charges and sizes under consideration. In agreement with these findings, the third equation is approximately consistent with experimental data on the condition that GPL is allowed to expand with decreasing ionic strength, with simultaneous decrease of its full charge density (sigma(f)). The results are compared with relevant evidence for erythrocytes. The possible applications of the inferences arrived at in electrophoretic studies of cell behaviour are also discussed.