Induction, purification and characterization of an antibacterial peptide scolopendrin I from the venom of centipede Scolopendra subspinipes mutilans.
Indian J Biochem Biophys
;
2006 Apr; 43(2): 88-93
Article
in English
| IMSEAR
| ID: sea-28151
ABSTRACT
The crude venom of the centipede Scolopendra subspinipes mutilans, injected with Escherichia coli K12D31 for 3-4 days showed broad-spectrum antimicrobial activity against Gram-positive. Gram-negative bacteria and fungi. It showed good antibacterial activity against E. coli K12D31 at different temperatures, pH, and ionic strengths. The crude venom was heated at 100 degrees C for 30 min, centrifuged at 10,000 rpm for 30 min at 4 degrees C and the supernatants were obtained, from which an antibacterial fraction having a molecular mass of 3000-5000 Da, was further separated by ultrafiltration. A homogeneous antibacterial peptide named scolopendrin I, having a molecular mass of 4,498 Da, was isolated using cation-exchange chromatography and two steps of reverse-phase high performance liquid chromatography (RP-HPLC). Scolopendrin I did not show any hemolytic and agglutination activities at the concentration below 30 microM.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Arthropod Venoms
/
Arthropods
/
Antimicrobial Cationic Peptides
/
Indoles
/
Animals
/
Anisoles
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS