Solubilization and binding of DNA-CTAB complex with SDS in aqueous media.

ABSTRACT

Extent of binding (gamma 2(1)) of sodium dodecyl sulphate (SDS) to the binary complex formed between calfthymus DNA and cetyltrimethylammonium bromide (CTAB) has been measured in mole per mole of nucleotide in the complex as function of concentration of SDS by using equilibrium dialysis technique at different temperatures and pH. Binding of SDS to thermally denatured DNA-CTAB complex has also been studied. The most interesting aspect to be noted in this experiment is that the water insoluble DNA-CTAB binary complex gets solubilized in the ternary mixture in presence of SDS but when DNA is thermally denatured, the ternary system DNA-CTAB-SDS remains insoluble. Significant change in the extent of binding has been noted with the variation of the relative composition of DNA and CTAB in their binary mixture. The data of binding of SDS to DNA-CTAB complex are compared more precisely in terms of the standard Gibbs' free energy decrease (-delta G degree) for the saturation of the binding sites in the complex with the change of SDS activity from zero to unity in the rational mole fraction scale.