Purification of an allergenic protein fraction from wheat grain.
Indian J Biochem Biophys
;
1998 Jun; 35(3): 189-92
Article
in English
| IMSEAR
| ID: sea-28176
ABSTRACT
An allergenic protein fraction was isolated from wheat gluten by ion exchange and gel filtration chromatography. On sodium dodecyl sulphate gel electrophoresis the molecular mass of the protein was found to be 65 kDa. By Western blotting it was confirmed that the 65 kDa protein was the major allergenic fraction causing dermatitis herpetiformis (DH) and it contained glutamic acid and proline as the major amino acids.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plant Proteins
/
Triticum
/
Allergens
/
Chromatography
/
Dermatitis Herpetiformis
/
Electrophoresis, Polyacrylamide Gel
/
Amino Acids
/
Glutens
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1998
Type:
Article
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