Control of morphogenesis in tobacco protoplast cultures: organogenesis vs embryogenesis.
Indian J Biochem Biophys
;
1991 Oct-Dec; 28(5-6): 467-71
Article
in English
| IMSEAR
| ID: sea-28263
ABSTRACT
The morphogenetic pathway leading to plant differentiation in tobacco mesophyll protoplasts could be regulated. The course of development via organogenesis or embryogenesis was controlled by manipulating nutrient media, culture conditions and hormone requirements. A lowering of molarity of medium after 5 weeks of protoplast culture, inclusion of GA3 (0.5 mg/l) in the medium for first 8 weeks of culture and exclusion of reduced nitrogen in the medium resulted in shoot organogenesis, while maintenance of higher molarity of the medium till 8 weeks, reduced nitrogen in the medium and removal of 2, 4-D after 5 weeks of culture induced embryogenesis. Regenerability of viable plants was obtained by both developmental pathways. The implications of tobacco embryogenesis system in plant molecular genetics were highlighted.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plants, Toxic
/
Protoplasts
/
Regeneration
/
Plant Physiological Phenomena
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1991
Type:
Article
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