NADH-dependent respiration in osmotically inactive swollen mitochondria: does transport replace phosphorylation in mediating respiratory control in swollen mitochondria?
Article
in English
| IMSEAR
| ID: sea-28348
ABSTRACT
Rotenone-sensitive, uncoupler-insensitive, NADH-dependent respiration was demonstrated in osmotically inactive fragments of the mitochondrial inner-membrane obtained following high amplitude (spontaneous) swelling. This NADH-dependent respiration as well as mitochondrial ATPase activity was stimulated by ligands which are known to be transported by specific transporters/mechanisms. The ligands capable of this anomalous respiratory control included several intermediates of the citric acid cycle, besides non-metabolizable ligands including lactate, cations such as K+ and Ca2+. The interaction between NADH-dependent respiration and these ligands, as manifested by stimulation of respiration, was strongly ionic strength-dependent. The thermodynamic relationship between respiratory control and stimulation of transport ATPase by the relevant transportable ligands could also be demonstrated in the conventional (rat liver) microsomes. These experimental results offer a novel experimental base for search into an intra-membranous mechanism of energy transduction.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Osmosis
/
Oxygen Consumption
/
Phosphorylation
/
Rats
/
Rats, Inbred Strains
/
Biological Transport, Active
/
Mitochondria, Liver
/
Animals
/
Mitochondrial Swelling
/
NAD
Language:
English
Year:
1991
Type:
Article
Similar
MEDLINE
...
LILACS
LIS