Isolation of Gln- mutants through transposon, Tn917, mutagenesis in Bacillus brevis.
Indian J Biochem Biophys
;
1991 Oct-Dec; 28(5-6): 472-5
Article
in English
| IMSEAR
| ID: sea-28380
ABSTRACT
Transposon, Tn917, carried on pTV1 plasmid has been used successfully to mutagenise Bacillus brevis. The transposon showed preference for insertion at an "aro" site. A second insertional event after elimination of the preferred site with ethidium bromide/acridine orange treatment has permitted isolation of Gln- mutants in B. brevis.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Bacillus
/
DNA Transposable Elements
/
Mutagenesis, Insertional
/
Glutamine
Language:
English
Journal:
Indian J Biochem Biophys
Year:
1991
Type:
Article
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