Effect of gamma-irradiation on H3 histone and DNA in solution.
Indian J Biochem Biophys
;
2001 Dec; 38(6): 406-11
Article
in English
| IMSEAR
| ID: sea-28731
ABSTRACT
Three methods, namely, absorbance of colour by reaction with Folin-Ciocalteau reagent, UV absorbance and fluorescence intensity measurements for detection of H3 histone in 0.15 M standard saline citrate (SSC) solution were compared. Maximum sensitivity was found with the Folin-Ciocalteau method. Effect of varying pH and of gamma- radiation on H3 histone and on interaction of H3 histone with DNA were studied. For this, solutions of H3 histone in SSC, in 0.9% NaCl, H3 histone + DNA in 0.9% NaCl were subjected to varying pH (1-10) and gamma- radiation (dose 10-50 Gy) and lambda(max) and Alambda(max) were monitored. From the molar ratios of histone and DNA in the complex, it was observed that at gamma -radiation dose of 50 Gy and pH 8.54, there was a depletion of 6-8 microg/ml of histone from the histone-DNA complex.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Spectrometry, Fluorescence
/
Spectrophotometry, Ultraviolet
/
Thymus Gland
/
DNA
/
Cattle
/
Histones
/
Tungsten Compounds
/
Gamma Rays
/
Animals
/
Molybdenum
Language:
English
Journal:
Indian J Biochem Biophys
Year:
2001
Type:
Article
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